Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection.
William Heard, Jan Sklenář, Daniel F. A. Tomé, Silke Robatzek and Alexandra M. E. Jones
Molecular & Cellular Proteomics (2015) 14: 1796-1813.
This paper investigates an approach to decipher the membrane compartmentalization, through which cells separate and control biological processes. Plant growth, development, and adaptation to biotic and abiotic stress all rely on the dynamic endomembrane system, yet there is little known about the proteins regulating these dynamic trafficking events.
The authors developed a method based on affinity purification of fluorescent fusion constructs to seven subcellular marker proteins in Arabidopsis thaliana seedlings. A total of 433 proteins were presented, only five of which were shared among all enrichments, while many proteins were common between endomembrane compartments of the same trafficking route. Approximately half were assigned to only one enrichment.
More details as well as datasets are available from the proteomics database PRIDE (PXD001877 to PXD1885 inclusive).