Targeting Synaptic Pathology with a Novel Affinity Mass Spectrometry Approach
Ann Brinkmalm, Gunnar Brinkmalm, William G. Honer, Julie A. Moreno, Joel Jakobsson,
Giovanna R. Mallucci, Henrik Zetterberg, Kaj Blennow, and Annika Öhrfelt.
Molecular & Cellular Proteomics October 1, 2014, Vol. 13, 2584-2592
This study presents a method for characterizing and quantifying proteins in the SNARE complex, which are involved in presynaptic regulation and may play a role in early stage dysfunction in Alzheimer's disease. These proteins are difficult to analyze by mass spectrometry as they are membrane-associated and strongly form complexes.
Combining affinity purification by immunoprecipitation and mass spectrometry, the authors showed reduced levels of the SNARE proteins in brain tissue from AD patients as well as in prion-infected mice. The particular advantage of the MS-based method over immunological assays is that all SNARE proteins, including modified forms, can be quantified simultaneously.